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Chuck receives an email from his old college roommate, Bryce Larkin, which contains government secrets from a computer known as the Intersect, that embed themselves into his brain.  The CIA and NSA both want the data back so they send in their agents, Sarah Walker and John Casey to retrieve the data.

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Chuck Season 1 Episode 1 Quotes

Sarah: Wow, I didn't think people still named their kids Chuck. Or Morgan, for that matter.
Chuck: My parents were sadists, and carnival freaks found him in a dumpster.
Morgan: But they raised me as one of their own!

Chuck: Uh, you know, Sis, the thing is, Morgan and I don't really feel like we're fitting my birthday party...'cause we don't know anybody, 'cause they're all your friends, and they all happen to be doctors.
Morgan: Doctors who don't really get our jokes!
Chuck: Well, your jokes